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  • Title: PilJ localizes to cell poles and is required for type IV pilus extension in Pseudomonas aeruginosa.
    Author: DeLange PA, Collins TL, Pierce GE, Robinson JB.
    Journal: Curr Microbiol; 2007 Nov; 55(5):389-95. PubMed ID: 17713814.
    Abstract:
    Twitching motility allows Pseudomonas aeruginosa to respond to stimuli by extending and retracting its type IV pili (TFP). PilJ is a protein necessary for this surface-associated twitching motility and bears high sequence identity with Escherichia coli methyl-accepting chemotaxis proteins (MCP). Here, we report that whereas wild-type P. aeruginosa PAO1 cells have extended pili at a single pole, pilJ mutant cells have shortened pili often at both poles despite normal levels of pilin accumulation, suggesting that PilJ is required for full TFP assembly/extension. Using yellow fluorescent protein fusions (pilJ-yfp), both plasmid born and in-frame chromosomal constructs, we determined that PilJ localizes to both poles of the cell. Overexpression of pilJ-yfp resulted in the protein accumulating between the poles.
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