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Title: Antimicrobial resistance of 114 porcine isolates of Campylobacter coli. Author: Shin E, Lee Y. Journal: Int J Food Microbiol; 2007 Sep 15; 118(2):223-7. PubMed ID: 17716763. Abstract: Campylobacter species were isolated from 24 pig farms in 10 different regions of Korea, and were assayed with regard to their susceptibility to eight antimicrobial agents. A total of 114 Campylobacter isolates from 572 intestinal samples were all identified as C. coli via both classical methods and molecular methods, including 16S rDNA sequence analysis and polymerase chain reactions (PCR) using specific primer sets for the hippuricase gene and the aspartokinase gene, designed to differentiate C. coli from C. jejuni. Minimal inhibitory concentrations of seven antimicrobial agents were determined via agar dilution: the MIC(90)s were 64 microg/ml for ampicillin, 8 microg/ml for chloramphenicol, 64 microg/ml for ciprofloxacin, 16 microg/ml for enrofloxacin, >or=128 microg/ml for erythromycin, >or=128 microg/ml for gentamicin, and >or=128 microg/ml for tetracycline. The proportion of isolates resistant to each antimicrobial agent was as follows: 28.9% for ampicillin, 2.6% for chloramphenicol, 84.2% for ciprofloxacin, 83.3% for enrofloxacin, 46.5% for erythromycin, 20.2% for gentamicin, and 56.1% for tetracycline. All 114 isolates were found to be resistant to at least one antimicrobial agent, and 61 isolates (53.5%) were found to be multi-drug resistant (resistant to more than three antimicrobial agents in different classes).[Abstract] [Full Text] [Related] [New Search]