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  • Title: KLF4 regulates the expression of interleukin-10 in RAW264.7 macrophages.
    Author: Liu J, Zhang H, Liu Y, Wang K, Feng Y, Liu M, Xiao X.
    Journal: Biochem Biophys Res Commun; 2007 Oct 26; 362(3):575-81. PubMed ID: 17719562.
    Abstract:
    Krüppel-like factor 4 (KLF4) is an evolutionarily conserved zinc finger-containing transcription factor. In the current study, RAW264.7 murine macrophages were treated with Escherichia coli lipopolysaccharide (LPS) to determine the expression of KLF4. A full-length cDNA or antisense oligonucleotides of KLF4 was transfected into RAW264.7 macrophages, and the expression and release of IL-10 were analyzed by RT-PCR and ELISA. The transcription and DNA binding activities of KLF4 to the IL-10 promoter were detected by the luciferase reporter and electrophoretic mobility shift assays. The results showed that treatment of RAW264.7 macrophages with LPS resulted in a dose- and time-dependent increase in KLF4 protein levels; KLF4 overexpression increased the expression of IL-10, while KLF4 inhibition decreased the expression of IL-10. The overexpression of KLF4 promoted the transcription and DNA binding activities of KLF4 to the IL-10 promoter. These results indicate that KLF4 plays an important role in regulating the expression of IL-10.
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