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  • Title: A simpler analysis for the measurement of second virial coefficients by self-interaction chromatography.
    Author: Winzor DJ, Scott DJ, Wills PR.
    Journal: Anal Biochem; 2007 Dec 01; 371(1):21-5. PubMed ID: 17723222.
    Abstract:
    We describe a thermodynamic approach that supports the adoption of a simplified procedure for the determination of protein second virial coefficients (B(2)) by self-interaction chromatography. Its major advantage over the original method is a decrease in the number of parameters to which magnitudes must be assigned for the determination of B(2). Improved correlation of virial coefficients obtained by the chromatographic procedure with those obtained by light scattering is achieved by taking into account the twofold larger magnitudes of the former because of the experimental distinction between free and immobilized protein molecules in self-interaction chromatography.
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