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  • Title: Evidence for the existence of cytoskeleton-bound polysomes in plants.
    Author: Davies E, Fillingham BD, Oto Y, Abe S.
    Journal: Cell Biol Int Rep; 1991 Oct; 15(10):973-81. PubMed ID: 1773445.
    Abstract:
    When conventional, high ionic strength buffers were used for the isolation of polysomes from pea plants, less than 20% were retained in the detergent-insoluble pellet. Reducing Tris, K+ and Mg++ to 10 mM increased retention to 70%, and when a new, microfilament-stabilizing buffer was used, retention increased to 80%. Conditions which favoured polysome pelleting at lower g forces permitted the retention of actin in the pellet. The data are consistent with the hypothesis that higher plants, like animals, contain cytoskeleton-(actin)-bound polysomes.
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