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  • Title: [Determination of berberine hydrochloride in traditional Chinese medicines containing Coptis chinensis Franch by reversed phase high performance liquid chromatography].
    Author: Guo P, Li Z, Hong Z, Liu S, Wu T.
    Journal: Hua Xi Yi Ke Da Xue Xue Bao; 1991 Mar; 22(1):90-2. PubMed ID: 1774045.
    Abstract:
    This paper reports a reversed phase high performance liquid chromatography method to determine berberine hydrochloride in Coptis chinensis Franch and in the traditional Chinese medicine containing Coptis chinensis Franch. The result shows that the mobile phase of 0.4mol/L ammonium nitratemethanol (52:48) can give a satisfactory separation. The extraction of sample with 1% H2SO4 solution using the ultrasonic vibration method is simple and easy to operate.
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