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  • Title: Identification of mycobacteria by high-performance liquid chromatography.
    Author: Butler WR, Jost KC, Kilburn JO.
    Journal: J Clin Microbiol; 1991 Nov; 29(11):2468-72. PubMed ID: 1774251.
    Abstract:
    Mycolic acids extracted from saponified mycobacterial cells were examined as p-bromophenacyl esters by high-performance liquid chromatography (HPLC). Standard HPLC patterns were developed for species of Mycobacterium by examination of strains from culture collections and other well-characterized isolates. Relative retention times of peaks and peak height comparisons were used to develop a differentiation scheme that was 98% accurate for the species examined. A rapid, cost-effective HPLC method which offers an alternative approach to the identification of mycobacteria is described.
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