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  • Title: Genotyping spring viraemia of carp virus and other piscine vesiculo-like viruses using reverse hybridisation.
    Author: Sheppard AM, Le Deuff RM, Martin PD, Woolford G, Way K, Stone DM.
    Journal: Dis Aquat Organ; 2007 Jun 29; 76(2):163-8. PubMed ID: 17760389.
    Abstract:
    A simple nylon membrane-based DNA macroarray was developed to genotype spring viraemia of carp virus (SVCV) and related viruses. Twenty-six viruses were genotyped using the array, and the results were confirmed by phylogenetic analysis of a 426 bp partial glycoprotein gene sequence. The array was not only capable of discriminating between the 4 main genogroups of cyprinid vesiculo-type viruses described previously, but also accurately sub-type the SVC viruses assigned to Genogroup I. The assay offers a practical solution for diagnostic laboratories that currently lack a sequencing capability to confirm the nature of PCR products generated in suspected SVCV cases.
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