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  • Title: [Surface-enhanced Raman spectra of oxidation damnification of fetal bovine serum by ozone].
    Author: Zou ZQ, Liu YN, Wu Y, Li QN, Chen MZ, Sun HY, Lee I.
    Journal: Guang Pu Xue Yu Guang Pu Fen Xi; 2007 Jun; 27(6):1140-2. PubMed ID: 17763777.
    Abstract:
    Fetal bovine serum was treated by ozone for 1 hour and 3 hours before getting its surface-enhanced Raman spectra from 200 to 1 800 cm(-1). Treated with ozone for 1 hour, it shows a significant decrease in band intensity. Treated with ozone for 3 hours, the band intensity has a further decrease but not so obviously, which means that oxidation of ozone is short lived. Treated with ozone, the orderly conformations of main chains in protein such as alpha-helix, beta-sheet and beta-corner are damaged seriously. Aromatic side chains and C-S of Cys and Met also are damnified greatly. All this means that strong oxidation of ozone results in denaturation, conformational changes and even degradation in protein.
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