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  • Title: Integrated digital image and accelerometer measurements of rat locomotor and vibratory behaviour.
    Author: Shih YH, Young MS.
    Journal: J Neurosci Methods; 2007 Oct 15; 166(1):81-8. PubMed ID: 17765321.
    Abstract:
    This study developed a combined IC-type accelerometer and video camera system to simultaneously measure vibration and locomotion activities in rats. A personal computer, adopted as an image frame grabber, was combined with a digital image processing algorithm to measure the precise location of an animal in an experimental cage. An accelerometer-based vibration subsystem, based on an 89C51 single-chip microprocessor, was designed. The acceleration sensor module was attached directly to the shaved back of the rat's body to directly measure the animal's vibration. This module can detect a wide range of vibrations from movements of the entire body to micro-tremors. Along with hardware, this study also proposes novel software for video enhancement and data analysis to calculate the behaviour parameters from recorded movements. In normal mode, three vibration activities (locomotor activity, tremor and twitch) are auto-analyzed every 10 min. The results are saved, and various display, statistical and data organization options are available. The primary merits of this system are the ability to simultaneously record locomotion and vibrational data, the rapid set-up and operation, the low cost, the reduced illumination requirements, the reduction of environmental noise and the high precision. The proposed method will be of interest to researchers in various behavioural, biological and medical fields.
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