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  • Title: An improved method for Organic Bound Tritium (OBT) determination in urine samples.
    Author: Gonen R, Marco R, German U, Katorza E, Alfassi ZB, Haquin G, Tshuva A, Pelled O.
    Journal: Radiat Prot Dosimetry; 2007; 125(1-4):460-4. PubMed ID: 17766260.
    Abstract:
    The Organic Bound Tritium (OBT) amount in the body may induce changes in the evaluation of the internal exposure to tritium, due to its different retention time relative to HTO. OBT measurements for urine are not performed routinely, mainly because of the lengthy work needed in preparation of the samples, when using the standard oxygen combustion method. A simpler and more rapid method based on liquid scintillation counting (LSC) was employed, and an evaluation was performed to check its suitability for urine samples. The principle of the proposed method is based on subtracting the LSC counts of the water phase sample (HTO) from the total activity of the urine sample (OBT+HTO). A good correlation was found between the results obtained when applying the proposed direct method and the Gold standard method. The minimum detectable activity when using this method was determined.
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