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  • Title: Assessment of two commercial agglutination kits for detecting Escherichia coli heat-labile enterotoxin.
    Author: Speirs J, Stavric S, Buchanan B.
    Journal: Can J Microbiol; 1991 Nov; 37(11):877-80. PubMed ID: 1777863.
    Abstract:
    Two commercial agglutination kits, a reserved passive agglutination test (VET-RPLA) and a staphylococcal coagglutination test (Phadebact ETEC-LT Test), were compared with two cell culture assays (Y-1 and Vero) and GM1 ganglioside enzyme-linked immunosorbent assay (GM1-ELISA) for sensitivity in detecting Escherichia coli heat-labile enterotoxin (LT). Of 48 toxigenic strains, 23 were positive by all assays. One strain was negative only by the Phadebact test. Four strains, all LT-II producers, were positive by cell culture only. For LT-I detection, the Phadebact test was the least sensitive but was simple and rapid; VET-RPLA was simple, sensitive, and a good substitute for cell culture or GM1-ELISA.
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