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  • Title: [Determination of three glycosides from herbs of Swertia punicea by RP-HPLC].
    Author: Zhang XQ, Huang FJ, Chen JC, Liu YW.
    Journal: Zhongguo Zhong Yao Za Zhi; 2007 Jun; 32(12):1184-6. PubMed ID: 17802883.
    Abstract:
    OBJECTIVE: To develop a RP-HPLC method for determination of three glycosides in Swertia punicea. METHOD: Chromatographic column: Alltimal C18 (4.6 mm x 250 mm, 5 microm). Mobile phase: methanol-water (including 0.05% H3PO4), and gradient elution. Flow rate: 1 mL x min(-1). Wavelength: 254 nm. Column temperture: 30 degrees C. RESULT: The calibration curves of gentiopicroside, mangiferin and swertrianolin were in good linearity over the range of 31.3-281.7, 0.31-2.78, 0.55-4.91 microg, (r = 0.9996, 0.9993, 0.9995). The average recoveries were 103.36%, 101.42% and 97.39%, with RSD less then 3% (n = 5). CONCLUSION: It is a simple and sensitive meathod in controlling the quality of S. punicea.
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