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  • Title: Immunoproteomic assay of membrane-associated proteins of Streptococcus suis type 2 China vaccine strain HA9801.
    Author: Zhang W, Lu CP.
    Journal: Zoonoses Public Health; 2007; 54(6-7):253-9. PubMed ID: 17803514.
    Abstract:
    Immunoproteomic approaches were undertaken to study the immunogenicity of the membrane-associated proteins of the Streptococcus suis type 2 (SS2) China vaccine strain HA9801. The membrane-associated proteins were enriched using the Triton X-114 extraction protocol and were analysed by two-dimensional gel electrophoresis (2-DE) and subsequent immunoblotting using the hyperimmune serum of SS2-HA9801-immunized specific pathogen free (SPF) minipigs. A total of 11 proteins were recognized, and the corresponding spots on a duplicate gel were excised and identified by MALDI-TOF MS.
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