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  • Title: Stoichiometric complexation of Streptomyces subtilisin inhibitor and subtilisin.
    Author: Narhi LO, Connor J, Rohde MF, McGinley MG, Arakawa T.
    Journal: J Protein Chem; 1991 Aug; 10(4):385-9. PubMed ID: 1781884.
    Abstract:
    Subtilisin (Sbt) and Streptomyces subtilisin inhibitor (SSI) were analyzed either alone or together using sodium dodecylsulfate (SDS)-polyacrylamide gel electrophoresis (PAGE). With all ratios of Sbt to SSI tested, the proteins formed a stoichiometric complex, and migrated abnormally at the top of the gel. Electroblotting and amino acid sequence analysis of the complex band showed both Sbt and SSI present at approximately equal molar ratios. When excess Sbt was present, it migrated as a free but still folded form slightly above the band corresponding to the complex. When excess SSI was present, it migrated as several species with molecular weights smaller than the intact form; in fact, the sequences of some of these species indicated that they lacked different amounts of N-terminal and possibly C-terminal residues.
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