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  • Title: Quantification of Y chromosome bearing spermatozoa of cattle using in situ hybridization.
    Author: Schwerin M, Blottner S, Thomsen PD, Roschlau D, Brockmann G.
    Journal: Mol Reprod Dev; 1991 Sep; 30(1):39-43. PubMed ID: 1781986.
    Abstract:
    An easy assay for quantification of Y chromosome-bearing sperm (Y-sperm) is needed, especially to monitor sperm separation techniques. In the present study a tritiated bovine male-specific DNA fragment was tested for identification of Y-sperm by in situ hybridization. A protocol for in situ hybridization to bovine sperm was developed and used to study the proportion of Y-sperm of 12 bulls. The usefulness of the method in optimization of sperm separation procedures is illustrated through analysis of fractions of sperm separated by Percoll density gradient centrifugation.
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