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  • Title: Quantitative analysis of biofilm EPS uronic acid content.
    Author: Mojica K, Elsey D, Cooney MJ.
    Journal: J Microbiol Methods; 2007 Oct; 71(1):61-5. PubMed ID: 17822791.
    Abstract:
    The uronic acids assay was evaluated for its ability to measure the amount of uronic acids contained within a biofilm exopolysaccharide matrix. Cytophaga lytica, a marine bacterium isolated from a naturally occurring biofilm, was used to form single-species biofilms for the method assessment. The assay was found to be simple, reproducible, and sensitive to 1 microg levels, suggesting its potential for application as a screening technique for compounds that inhibit the production of microbial biofilm exopolysaccharide containing uronic acids.
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