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  • Title: Measurement of alkaline phosphatase activity in serum with N-methyl-D-glucamine as a buffer: evaluation of the method for routine use.
    Author: Franzini C, Cattozzo G, Ceriotti F, Ferrero CA, Berti P, Bianchi P, Bonvicini P, Ceriotti G, Deledda R, Fenili D.
    Journal: Eur J Clin Chem Clin Biochem; 1991 Nov; 29(11):759-65. PubMed ID: 1782284.
    Abstract:
    Some aspects of the measurement of alkaline phosphatase activity concentration in human serum, using N-methyl-D-glucamine as a buffer, were evaluated with a view to the possible routine use of the method. The evaluated characteristics included: the temperature-dependence of the pH of the buffer; the effect of adding the magnesium/zinc ions buffer; the effect of the serum volume fraction; the substrate-starter versus the serum-starter mode; the effect of modifying the formulation of reagents; the within-run and the between laboratory imprecision; the correlation with an alternative routine method. Also, sex- and age-related reference values were produced, based on 2968 values from selected reference sample groups in 7 laboratories. In general, the results demonstrate the robustness of the method, its adaptability to a variety of mechanized analysers, and hence its feasibility as a routine measurement method.
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