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  • Title: International Federation of Clinical Chemistry (IFCC), scientific division: IFCC recommendation on sampling transport and storage for the determination of the concentration of ionized calcium in whole blood, plasma and serum.
    Author: Boink AB, Buckley BM, Christiansen TF, Covington AK, Maas AH, Müller-Plathe O, Sachs C, Siggaard-Andersen O.
    Journal: Eur J Clin Chem Clin Biochem; 1991 Nov; 29(11):767-72. PubMed ID: 1782285.
    Abstract:
    The substance concentration of ionized calcium (cCa2+) in blood, plasma or serum preanalytically may be affected by pH changes of the sample, calcium binding by heparin, and dilution by the anticoagulant solution. pH changes in whole blood can be minimized by anaerobic sampling to avoid loss of CO2, by measuring as soon as possible or by storing the sample in iced water to avoid lactic acid formation. cCa2+ and pH should be determined simultaneously.
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