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  • Title: Liquid chromatography tandem mass spectrometry method for simultaneous determination of metoprolol tartrate and ramipril in human plasma.
    Author: Gowda KV, Mandal U, Senthamil Selvan P, Sam Solomon WD, Ghosh A, Sarkar AK, Agarwal S, Nageswar Rao T, Pal TK.
    Journal: J Chromatogr B Analyt Technol Biomed Life Sci; 2007 Oct 15; 858(1-2):13-21. PubMed ID: 17825632.
    Abstract:
    A simple, rapid, sensitive and specific liquid chromatography-tandem mass spectrometry method was developed and validated for quantification of metoprolol tartrate (MT) and ramipril, in human plasma. Both the drugs were extracted by liquid-liquid extraction with diethyl ether-dichloromethane (70:30, v/v). The chromatographic separation was performed on a reversed-phase C8 column with a mobile phase of 10 mM ammonium formate-methanol (3:97, v/v). The protonated analyte was quantitated in positive ionization by multiple reaction monitoring with a mass spectrometer. The method was validated over the concentration range of 5-500 ng/ml for metoprolol and ramipril in human plasma. The precursor to product ion transitions of m/z 268.0-103.10 and m/z 417.20-117.20 were used to measure metoprolol and ramipril, respectively.
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