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  • Title: Application of Plackett-Burman experimental design and Doehlert design to evaluate nutritional requirements for xylanase production by Alternaria mali ND-16.
    Author: Li Y, Liu Z, Cui F, Liu Z, Zhao H.
    Journal: Appl Microbiol Biotechnol; 2007 Nov; 77(2):285-91. PubMed ID: 17846761.
    Abstract:
    The objective of this study was to use statistically based experimental designs for the optimization of xylanase production from Alternaria mali ND-16. Ten components in the medium were screened for nutritional requirements. Three nutritional components, including NH(4)Cl, urea, and MgSO(4), were identified to significantly affect the xylanase production by using the Plackett-Burman experimental design. These three major components were subsequently optimized using the Doehlert experimental design. By using response surface methodology and canonical analysis, the optimal concentrations for xylanase production were: NH(4)Cl 11.34 g L(-1), urea 1.26 g L(-1), and MgSO(4) 0.98 g L(-1). Under these optimal conditions, the xylanase activity from A. mali ND-16 reached 30.35 U mL(-1). Verification of the optimization showed that xylanase production of 31.26 U mL(-1) was achieved.
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