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  • Title: The effect of insulin and relaxin upon mitosis (in vitro) in mammary tissue from pregnant and lactating pigs.
    Author: Buttle HL, Lin CL.
    Journal: Domest Anim Endocrinol; 1991 Oct; 8(4):565-71. PubMed ID: 1786703.
    Abstract:
    The rate of cellular proliferation in the mammary glands of pigs during late gestation and lactation was assessed by measuring the incorporation of 3H-thymidine (T1) into the DNA of mammary gland explants in vitro. The T1 showed a linear response over the first 9 hr in vitro, and was not affected by the addition of 500 ng insulin/ml medium. From day 100 to parturition the T1 rose, reached a peak at 2 d after parturition and declined during lactation to the lowest levels seen at day 21 of lactation. The inclusion of 0-1000 ng relaxin/ml medium on T1 at 24-72 hr in vitro had no effect in stimulating T1 in mammary tissue explants taken from either pregnant or lactating pigs.
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