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  • Title: An aminoglycoside microarray platform for directly monitoring and studying antibiotic resistance.
    Author: Disney MD, Barrett OJ.
    Journal: Biochemistry; 2007 Oct 09; 46(40):11223-30. PubMed ID: 17867707.
    Abstract:
    Antibiotic resistance is a major threat to human health. Since resistance to the aminoglycoside class of antibiotics is most commonly caused by enzymatic modification, we developed a high-throughput microarray platform for directly assaying resistance enzyme activity on aminoglycosides. After modification, the array can be hybridized with the therapeutic target, a bacterial rRNA A-site mimic, to study the effect that modification has on binding. Such studies will help identify important factors that contribute to high-affinity recognition of therapeutic targets and low-affinity recognition of and modification by resistance enzymes. This platform may also be useful for screening chemical libraries to discover new antibiotics that evade resistance.
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