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Title: Pneumocystis carinii pneumonia: detection of parasites by immunofluorescence based on a monoclonal antibody. Author: Magee JG, McDade KJ, Cunningham J, Harrison V. Journal: Med Lab Sci; 1991 Jul; 48(3):235-7. PubMed ID: 1787784. Abstract: A monoclonal antibody-based immunofluorescence test for the detection of Pneumocystis carinii was evaluated in comparison with the conventional direct staining by Grocott's silver methenamine technique. A total of 254 respiratory samples from HIV positive and other immunocompromised patients were examined. Cysts were detected in 30 (12%) of samples using the monoclonal test, but only 15 (6%) positives were found using the Grocott method. There is need for a speedy and efficient test for detection of these organisms, and the monoclonal test was found to be more reliable, quicker and more sensitive than the Grocott technique.[Abstract] [Full Text] [Related] [New Search]