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  • Title: Prototype single step lateral flow technology for detection of avian influenza virus and chicken antibody to avian influenza virus.
    Author: Nielsen K, Yu WL, Lin M, Davis SA, Elmgren C, Mackenzie R, Tanha J, Li S, Dubuc G, Brown EG, Keleta L, Pasick J.
    Journal: J Immunoassay Immunochem; 2007; 28(4):307-18. PubMed ID: 17885885.
    Abstract:
    A rapid and effective lateral flow assay (LFA) for detection of avian influenza virus (AIV) was developed. For antigen capture, the assay used monoclonal antibody specific for a conserved nuclear protein (NP) epitope, immobilized on a cellulose acetate matrix, in conjunction with a second NP monoclonal antibody chemically linked to either coloured latex beads or colloidal gold particles contained in a sample pad for detection. Virus sample added to the sample pad flowed into the trapping antibody to form a visible band as well as a second, control band further along the acetate strip. The control band consisted of recombinant protein A/G, also immobilized on the matrix. A second LFA for detection of chicken antibody to AIV was developed where NP antigen was immobilized on the matrix with recombinant protein A/G immobilized as a control band. Latex beads or colloidal gold particles to which monoclonal anti-chicken antibody was attached, were used as the indicator system.
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