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  • Title: Translation of double-stranded RNA segments 1 and 2 of Chuzan virus in rabbit reticulocyte lysates.
    Author: Yoshida T, Harasawa R, Goto Y.
    Journal: Zentralbl Veterinarmed B; 1991 Oct; 38(8):630-4. PubMed ID: 1789019.
    Abstract:
    Coding assignments of genome segments 1 and 2 of Chuzan virus strain K-47 were studied in vitro by using rabbit reticulocyte lysates. The double-stranded RNA segment was extracted from sodium dodecyl sulfate (SDS)-polyacrylamide gels by a modified crushing and eluting technique. Translation products labeled with [35S]methionine were resolved by SDS-polyacrylamide gel electrophoresis. The molecular weights of the products from RNA segments 1 and 2 were estimated to be 98 and 95 kilodaltons, respectively.
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