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  • Title: Isolation and structure elucidation of thiazomycin- a potent thiazolyl peptide antibiotic from Amycolatopsis fastidiosa.
    Author: Jayasuriya H, Herath K, Ondeyka JG, Zhang C, Zink DL, Brower M, Gailliot FP, Greene J, Birdsall G, Venugopal J, Ushio M, Burgess B, Russotti G, Walker A, Hesse M, Seeley A, Junker B, Connors N, Salazar O, Genilloud O, Liu K, Masurekar P, Barrett JF, Singh SB.
    Journal: J Antibiot (Tokyo); 2007 Sep; 60(9):554-64. PubMed ID: 17917238.
    Abstract:
    Thiazolyl peptides are a class of rigid macrocyclic compounds richly populated with thiazole rings. They are highly potent antibiotics but none have been advanced to clinic due to poor aqueous solubility. Recent progress in this field prompted a reinvestigation leading to the isolation of a new thiazolyl peptide, thiazomycin, a congener of nocathiacins. Thiazomycin possesses an oxazolidine ring as part of the amino-sugar moiety in contrast to the dimethyl amino group present in nocathiacin I. The presence of the oxazolidine ring provides additional opportunities for chemical modifications that are not possible with other nocathiacins. Thiazomycin is extremely potent against Gram-positive bacteria both in vitro and in vivo. The titer of thiazomycin in the fermentation broth was very low compared to the nocathiacins I and III. The lower titer together with its sandwiched order of elution presented significant challenges in large scale purification of thiazomycin. This problem was resolved by the development of an innovative preferential protonation based one- and/or two-step chromatographic method, which was used for pilot plant scale purifications of thiazomycin. The isolation and structure elucidation of thiazomycin is herein described.
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