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  • Title: Comparison of spindle and chromosome configuration in in vitro- and in vivo-matured mouse oocytes after vitrification.
    Author: Huang JY, Chen HY, Park JY, Tan SL, Chian RC.
    Journal: Fertil Steril; 2008 Oct; 90(4 Suppl):1424-32. PubMed ID: 17919603.
    Abstract:
    OBJECTIVE: To compare the cytogenetic changes in in vitro- and in vivo-matured oocytes after vitrification. DESIGN: In vitro experiments using murine model. SETTING: Animal model study in university laboratory. ANIMAL(S): CD-1 mice. INTERVENTION(S): In vitro maturation and vitrification of oocytes. MAIN OUTCOME MEASURE(S): Post-warming survival, analysis of spindle and chromosome configurations, aneuploidy screening of parthenogenetically activated oocytes, extent of DNA fragmentation, and early embryonic development after IVF. RESULT(S): Eighty percent of germinal vesicle-stage oocytes matured after in vitro maturation and were cryopreserved by vitrification (n = 354). There was no significant difference in the post-warming survival of in vitro- and in vivo-matured oocytes (94.1% vs. 91.8%, respectively). The majority of in vitro- and in vivo-matured oocytes maintained normal meiotic spindle morphology and chromosome alignment (88.2% vs. 86.9%, respectively) after vitrification and the incidence of aneuploidy was not increased (11.5% vs. 9.3%). However, in vitro-matured oocytes showed a higher rate of DNA fragmentation after vitrification compared to in vivo-matured oocytes. After vitrification, the cleavage and blastocyst formation rates of in vitro-matured oocytes were significantly lower than those of in vivo-matured oocytes (37.0% vs. 60.0% and 5.4% vs. 18.9%, respectively). CONCLUSION(S): Vitrification of in vitro-matured mouse oocytes results in high survival rates, normal meiotic spindle and chromosome alignment, and no increased incidence of aneuploidy. A possible cause of the reduced developmental competence of in vitro-matured and vitrified oocytes may be due to DNA fragmentation.
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