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  • Title: An immunonanogold resonance scattering spectral probe for rapid assay of human chorionic gonadotrophin.
    Author: Jiang ZL, Zou MJ, Liang AH.
    Journal: Clin Chim Acta; 2008 Jan; 387(1-2):24-30. PubMed ID: 17936256.
    Abstract:
    BACKGROUND: Several assays for human chorionic gonadotrophin (hCG) such as radioimmunoassay and fluorescence immunoassays were reported. So far, there was no report about the immunonanogold resonance scattering spectral (ING-RSS) assay based on the immunoreaction and resonance scattering (RS) effect. METHODS: Three ING-RSS probes for hCG were prepared, using nanogold in size of 8, 10, 13 nm to label rabbit hCG antiserum (anti-hCG). RESULTS: In citric acid-Na(2)HPO(4) buffer solution and in the presence of polyethylene glycol (PEG)6000, the immunoreaction between hCG and nanogold-labeled anti-hCG took place, the immunonanogold-complex was formed and deposited. The decreased intensity DeltaI(RS) for the nanogold in sizes of 8, 10, 13 nm was proportional to hCG concentration in the ranges of 40-10000, 40-10000 and 40-8000 mIU/ml, with the detection limits of 19.4, 15.1 and 13.6 mIU/ml, respectively. The hCG in urine samples were assayed by the ING-RSS assay, and by immunospectrophotometry. The results of both assays showed a close correlation. CONCLUSION: This assay has simplicity, sensitivity and good selectivity for quantitative determination of hCG.
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