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  • Title: Analysis of underivatized polyamines by reversed phase liquid chromatography with electrospray tandem mass spectrometry.
    Author: Häkkinen MR, Keinänen TA, Vepsäläinen J, Khomutov AR, Alhonen L, Jänne J, Auriola S.
    Journal: J Pharm Biomed Anal; 2007 Nov 30; 45(4):625-34. PubMed ID: 17945452.
    Abstract:
    A reversed phase liquid chromatography-electrospray ionization-tandem mass spectrometric method (RP-LC-ESI-MS/MS) was developed to separate and detect polyamines with minimal sample pre-treatment and without any derivatization. Prior to MS/MS analysis, a complete chromatographic separation of polyamines was achieved by a linear gradient elution using heptafluorobutyric acid as a volatile ion-pair modifier, and signal suppression was prevented by post-column addition of 75% propionic acid in isopropanol to the column flow. The developed method was successfully applied to the identification of metabolites formed from N(1),N(12)-diethylspermine in the reaction catalyzed by recombinant human polyamine oxidase and to the detection of eight different polyamines in a standard mixture.
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