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  • Title: Mineralization of CaCO3 in the presence of egg white lysozyme.
    Author: Voinescu AE, Touraud D, Lecker A, Pfitzner A, Kunz W, Ninham BW.
    Journal: Langmuir; 2007 Nov 20; 23(24):12269-74. PubMed ID: 17949115.
    Abstract:
    The influence of egg white lysozyme on the size, shape, crystallography, and chemical composition of amorphous calcium carbonate (ACC) particles obtained from aqueous CaCl2-dimethyl carbonate (DMC)-NaOH solutions was studied. At the onset of precipitation, the presence of lysozyme led to much smaller particles (50-400 nm spherical amorphous lysozyme-calcium carbonate particles (Ly-ACC)) than those obtained from lysozyme-free solution. The nanospheres were in some cases aggregated and in addition embedded in a faint network. Their size and interconnection depended on the concentration of egg white lysozyme. When the Ly-ACC particles were left in contact with the mother liquor (CaCl2/DMC/NaOH/lysozyme solution) for 24 h, they transformed directly and exclusively into crystalline calcite. The observed results may be of relevance for a better understanding of the role of lysozyme in the process of eggshell mineralization.
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