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  • Title: [Effect of chitosan on the level of ROS in HepG2 cell induced by cadmium chloride].
    Author: Xie H, Wang J, Fan G, Wu Z.
    Journal: Wei Sheng Yan Jiu; 2007 Jul; 36(4):438-9. PubMed ID: 17953209.
    Abstract:
    OBJECTIVE: To study the effect of chitosan on ROS levels in HepG2 cell induced by cadmium chloride. METHODS: The experiments were divided into 6 groups: normal control group, cadmium chloride group (20micromol/L), chitosan group (0.50mg/ml) and three combined treatment group (cadmium chloride 20micromol/L and chitosan 0.02mg/ml, 0.10mg/ml and 0.50mg/ml). The HepG2 cells were treated by chitosan and cadmium chloride simultaneously for 4h. The fluorescence of 2', 7'-dichlorofluorescin (DCF) was measured by floweytometry as a mean of estimating the formation of reactive oxygen species (ROS). RESULTS: In the comparison with normal control, the DCF intensity of chitosan group only exhibited somewhat decrease (P > 0.05) and significantly increased in cadmium chloride group (20micromol/L) (P < 0.01). The DCF intensity decreased in all three combined groups were more higher than those of cadmium chloride group, and when the concentration of chitosan improved from 0.01mg/ml to 0.50mg/ml, the DCF intensity decreased from 275.593 to 174.597 and the DCF intensity significant decrease was observed at concentration of 0.10mg/ml and 0.50mg/ml chitosan. CONCLUSION: It was suggested that chitosan could antagonize the increase of ROS in HepG2 cell induced by cadmium chloride.
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