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  • Title: [Simultaneous determination of nine beta2-agonists residues in urines by ultra performance liquid chromatography-electrospray tandem mass spectrometry].
    Author: Jia X, Qu J, Meng J, Shao B.
    Journal: Wei Sheng Yan Jiu; 2007 Jul; 36(4):489-92. PubMed ID: 17953220.
    Abstract:
    OBJECTIVE: To study the determination method of nine beta2-agonists residues in urine. METHODS: Urine samples were duconjugated with beta-glucuronidase/arylsulfatase enzyme in acetate buffer and deproteinized by perchloric acid, and then adjusted pH4.0. Sample concentration and purification were performed by Oasis HLB, Oasis MCX. The separation was performed on a Waters ACQUITY UPLCTM BEH C18 column (100mm x 2.1mm i.d., 1.7microm) with gradient elution using methanol and water (containing 0.1% formic acid) at a flow rate of 0.3ml/min. RESULTS: The limits of detection (LOD) of the method were from 0.002 to 0.025ng/ml and the limits of quantification (LOQ) ranged from 0.007 to 0.08ng/ml. Average recoveries for nine beta2-agonists at the spiking levels of 0.1, 0.5 and 2ng/ml ranged from 77.4% to 101.7% with relative standard deviations between 3.4% and 18% . CONCLUSION: The method can be used to determine the residues of nine beta2-agonists in urines.
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