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  • Title: [Detecting VA, VD3, VE and beta-carotene with HPLC in human serums].
    Author: Zou XL, Zeng HY, Li YQ, Li XX, Zhang SW, Zheng B, Huang CY.
    Journal: Sichuan Da Xue Xue Bao Yi Xue Ban; 2007 Sep; 38(5):879-81. PubMed ID: 17953383.
    Abstract:
    OBJECTIVE: To develop a method of detecting retinol (vitamin A), vitamin D3, alpha-tocopherol (vitamin E) and beta-carotene in human serums with HPLC. METHODS: Proteins were precipitated with anhydrous ethanol. Fat-solutable vitamins in human serums were extracted with aether/petroleum ether mixture and determined with HPLC. RESULTS: The linear ranges for the retinol, VD3, alpha-tocopherol and beta-carotene were 0.012 microg/mL-500 microg/mL, 0.030 microg/mL-500 microg/mL, 0.12 microg/mL-500 microg/mL, and 0.015 microg/mL-500 microg/mL, respectively. The detection limits for the retinol, VD3, alpha-tocopherol and beta-carotene were 0.012 microg/mL, 0.030 microg/mL, 0.12 microg/mL and 0.015 microg/mL, respectively. The relative standard derivations (RSD) for the retinol, VD3, alpha-tocopherol and beta-carotene were 0.75%, 0.54%, 2.06% and 2.74%, respectively. The proposed method recovered 92%-116%, 98%-112%, 84.8%-106%, and 90%-105% retinol, VD3, alpha-tocopherol and beta-carotene in human serums respectively. CONCLUSION: The method is simple, quick and applicable to all of the four fat-solutable vitamins.
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