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  • Title: Differential interleukin-6 messenger ribonucleic acid expression and its distribution pattern in eutopic and ectopic endometrium.
    Author: Salmassi A, Acil Y, Schmutzler AG, Koch K, Jonat W, Mettler L.
    Journal: Fertil Steril; 2008 May; 89(5 Suppl):1578-84. PubMed ID: 17953945.
    Abstract:
    OBJECTIVE: To evaluate the concentration and distribution pattern of interleukin-6-producing cells in ectopic and eutopic endometrium. DESIGN: Collection of both tissues at the proliferative cycle phase. SETTING: A university endocrinology and laparoscopy program. PATIENT(S): Twelve samples from six women were obtained at operative laparoscopy in the proliferative phase of each woman's cycle. INTERVENTION(S): Paired sampling of ectopic (n = 6) and eutopic endometrium (n = 6). MAIN OUTCOME MEASURE(S): Concentration of IL-6 messenger RNA (mRNA) was measured by real-time RT-PCR, and its distribution was determined by in situ RT-PCR. RESULT(S): In vitro RT-PCR analysis showed distinct bands at 234 bp and 115 bp for IL-6 mRNA in eutopic and ectopic endometrium and in placenta as positive control. Real-time RT-PCR indicated significantly higher expression of IL-6 in tissue with endometriosis than in tissue without. In all 12 samples subjected to in situ RT-PCR, positive signals of IL-6 mRNA were observed in cytoplasm of epithelial and stroma cells of ectopic and eutopic endometrium tissue. CONCLUSION(S): The significant IL-6 mRNA increase in endometriosis compared with in eutopic endometrium may play a role in the development of endometriosis and may provide new directions for endometriosis research.
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