These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [A study on the apoptosis of gastric carcinoma cells induced by arsenic trioxide combined with Ad-IkappaBalphaM].
    Author: Liu BR, Hu LH, Guan JM, Liu D, Jiang HC.
    Journal: Zhonghua Nei Ke Za Zhi; 2007 Jul; 46(7):569-72. PubMed ID: 17959082.
    Abstract:
    OBJECTIVE: To study the activation of nuclear factor-kappaB (NF-kappaB) in gastric carcinoma SGC-7901 cells after treating with As(2)O(3) and the enhancement of the therapeutic effect of As(2)O(3) with recombinant adenovirus IkappaBalphaM. METHODS: Culture of gastric carcinoma SGC-7901 cells was carried out. The cells both uninfected and infected with Ad-IkappaBalpha but not treated with As(2)O(3) were used as control. Electrophoretic mobility shift assay (EMSA) and immunohistochemistry were used to detect the activation of NF-kappaB in the cells after treatment of As(2)O(3) and the combination with Ad-IkappaBalphaM. MTT, Hoechst staining and TUNEL were used to assay the change of apoptosis induced by As(2)O(3) after infection with Ad-IkappaBalphaM. RESULTS: The results of EMSA and immunohistochemical method showed that after the treatment of As(2)O(3) the cells showed high activity of NF-kappaB. Simultaneous infection with Ad-IkappaBalphaM can inhibit the activation of NF-kappaB; MTT method indicated that after the treatment of As(2)O(3) infected with Ad-IkappaBalphaM apoptosis rate of the cells (59.2 +/- 2.5)% was higher than that of the cells treated with As(2)O(3) and infected with Ad-IkappaBalpha but not treated with As(2)O(3) (47.5 +/- 2.3)% and these neither infected nor treated (40.0 +/- 1.2%), P < 0.01. The result of Hoechst staining method indicated that, in the group of cells treated with As(2)O(3) and infected with Ad-IkappaBalphaM, apoptosis rate is (27.7 +/- 2.6)%, which was higher than the that of the cells infected with Ad-IkappaBalpha (18.3 +/- 1.5)% but not treated with As(2)O(3) and these neither infected nor treated (11.0 +/- 1.7%), P < 0.05. Hoechst staining method was in accordance with TUNEL technique; it was shown that in the group of cells treated with As(2)O(3) and infected with Ad-IkappaBalphaM, apoptosis rate was (31.1 +/- 2.5)%, being still higher than that of cells infected with Ad-IkappaBalpha but not treated with As(2)O(3) (20.7 +/- 2.1)% and these neither infected nor treated (13.0 +/- 1.7)%, P < 0.01. Therefore, infection with Ad-IkappaBalphaM can significantly increase the apoptosis induced by As(2)O(3). CONCLUSIONS: Gastric carcinoma cells treated with As(2)O(3) show activity of NF-kappaB. It is indicated that the activity of NF-kappaB may be the mechanism of the antagonism of gastric carcinoma cells against the apoptosis induced by As(2)O(3). Infection with Ad-IkappaBalphaM can effectively inhibit the activation of NF-kappaB in gastric carcinoma cells and increase the cell apoptosis induced by As(2)O(3).
    [Abstract] [Full Text] [Related] [New Search]