These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Electrospray mass spectrometric characterization of hemoglobin Q (Hb Q-India) and a double mutant hemoglobin S/D in clinical samples.
    Author: Mandal AK, Bisht S, Bhat VS, Krishnaswamy PR, Balaram P.
    Journal: Clin Biochem; 2008 Jan; 41(1-2):75-81. PubMed ID: 17959160.
    Abstract:
    OBJECTIVES: The clinical analysis of hemoglobin by ion exchange chromatography can result in ambiguities in identification of the nature of the globin chain present in patient samples. LC/ESI-MS provides rapid and precise determination of globin chain masses. DESIGN AND METHODS: Hemolysate of hemoglobin Q-India and hemoglobin S/D/F have been analyzed using ESI-MS. Tandem-MS has been used to establish mutation in alpha chain of hemoglobin Q. RESULTS: The identification of hemoglobin Q-India is readily achieved by LC/ESI-MS, which establishes the presence of a mutant alpha chain differing in mass from normal alpha chain by 22 Da. The site of mutation has been identified by tandem-MS analysis of a tryptic fragment encompassing residues alphaV62-K90. LC/ESI-MS screening has also provide an example of simultaneous occurrence of mutant globin chains containing beta6E-->V (Hb S, sickle) and beta121E-->Q (Hb D) variant. Expression of gamma(G) globin chain is also demonstrated in this sample. CONCLUSIONS: The site of mutation in hemoglobin Q-India is identified as alpha64D-->H which differs from mutations alpha74D-->H in Hb Q-Thailand and alpha75D-->H in Hb Q-Iran. Mass spectrometric analysis of hemoglobins from a patient and her parents suggests inheritance of mutant beta globin genes from both parents.
    [Abstract] [Full Text] [Related] [New Search]