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  • Title: Localization of enzymes in specialized regions of the microsomal membrane.
    Author: Winqvist L, Dallner G.
    Journal: Biochim Biophys Acta; 1976 Jun 17; 436(2):399-412. PubMed ID: 179597.
    Abstract:
    Microsomal vesicles were centrifuged through sucrose density gradients containing deoxycholate. With 0.15% detergent electron transport enzymes and phosphatases could be separated. Increasing the deoxycholate concentration to 0.19% resulted in separation of the microsomal material into five bands containing (in order from the top of the gradient) adenosine monophosphatase, inosine diphosphatase and some glucose-6-phosphatase (band 1); NADH-linked (band 2) and NADH-linked (band 3) electron transport enzymes; and glucose-6-phosphatase (bands 4 and 5). It appears that enzymes are arranged in specialized patches in the microsomal membrane.
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