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  • Title: Mixer-settler counter-current chromatography with multiple spiral disk assembly.
    Author: Ito Y, Clary R, Sharpnak F, Metger H, Powell J.
    Journal: J Chromatogr A; 2007 Nov 23; 1172(2):151-9. PubMed ID: 17961580.
    Abstract:
    A novel system for performing high-speed counter-current chromatography has been developed for separation of biopolymers using polymer phase systems. The spiral disk assembly consisting of eight units, each equipped with over 300 mixer-settler sets, was constructed and performance evaluated in terms of retention of the stationary phase and separation efficiency. A series of experiments was performed with a polymer phase system composed of polyethylene glycol 1000 (12.5%, w/w) and dibasic potassium phosphate (12.5%, w/w) using two stable protein samples of myoglobin and lysozyme at various experimental conditions of flow rates and revolution speeds. The best results were obtained with revolution speeds of 800-1000rpm at flow rates of 0.25-0.5ml/min where the partition efficiency of several 100 theoretical plates was achieved with over 50% stationary phase retention.
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