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Title: [Use of snake venom immobilized phosphodiesterase for analysis of the nucleotide composition of oligodesoxyribonucleotides]. Author: Kagramanova VK, Aukati MF, Fedorova GV, Shabarova ZA. Journal: Biokhimiia; 1976 Feb; 41(2):394-7. PubMed ID: 179617. Abstract: A simple one-step technique for the analysis of nucleotide composition of oligodeoxyribonucleotides is described. The method is based on enzymatic hydrolysis and subsequent separation of hydrolysate on a "combined" column with Aminex A 28 anionite, on which top immobilyzed on DEAE cellulose snake venom phosphodiesterase is layed. The method is tested using a number of synthetic di- and tri-deoxyribonucleotides.[Abstract] [Full Text] [Related] [New Search]