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  • Title: [Evaluation of the transcription of locus rfb for the biosynthesis of LPS in Leptospira interrogans subtype hardjoprajitno].
    Author: Casas EM, Ríos RR, Bulach D, Adler B, de la Peña-Moctezuma A.
    Journal: Rev Cubana Med Trop; 2005; 57(1):43-4. PubMed ID: 17966475.
    Abstract:
    Plasmid pGL4W15-96 was constructed from the pGL4W74 vector without promoter for kanamycin and a sequence of 480pb containing the supposed J15 promoter with the objective of confirming the role of J15 sequence as a promoter for Leptospira, which restored the transcription of the gene of resistance to kanamycin in Escherichia coli and Leptospira biflexa, corroborating this way the function of the insertion as a promoter for both organisms.
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