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  • Title: Production of fructooligosaccharides by crude enzyme preparations of beta-fructofuranosidase from Aureobasidium pullulans.
    Author: Yoshikawa J, Amachi S, Shinoyama H, Fujii T.
    Journal: Biotechnol Lett; 2008 Mar; 30(3):535-9. PubMed ID: 17968507.
    Abstract:
    Fructooligosaccharides (FOS) were produced from sucrose by using crude enzyme preparations of beta-fructofuranosidases (FFases) obtained from sucrose-cultured cells of Aureobasidium pullulans DSM 2404. When the preparation mainly consisted of FFase I, that has high transfructosylating activity, the FOS yield was 62%. When the reaction was carried out with additional commercial glucose isomerase (GI) at an activity ratio of FFase and GI of 1:2, the maximum FOS yield reached 69%. This value was higher than those obtained previously using other Aureobasidium spp. (53-59%).
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