These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Isolation and identification of the mixture of L-hydroxyproline oligo-peptides by reversed-phase high performance liquid chromatography and electrospray ionization tandem mass spectrometry].
    Author: Sun Y, Lu K, Ma L, Cao S, Zhao Y.
    Journal: Se Pu; 2007 Jul; 25(4):524-7. PubMed ID: 17970111.
    Abstract:
    A reversed-phase high performance liquid chromatographic-electrospray ionization tandem mass spectrometric method (RP-HPLC/MS2) was developed for the analysis and identification of a reaction mixture of L-hydroxyproline oligo-peptides. The mixture was separated on a YWG C8 column (10 microm, 250 mm x 10 mm) using acetonitrile-water containing 0.06% trifluoroacetic acid (2:98, v/v) as the mobile phase. The separation conditions were optimized. The mixture was analysed with electrospray ionization mass spectrometry (ESI-MS) and ESI-MS/MS in positive mode. MS and MS2 spectra of individual chromatographic peak were obtained. It indicated that there were hydroxyproline dipeptide, hydroxyproline tripeptide and hydroxyproline cyclo-dipeptide in the mixtures, besides hydroxyproline. The described method can be effectively applied to perform identification of hydroxyproline oligo-peptide mixtures.
    [Abstract] [Full Text] [Related] [New Search]