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  • Title: Phospholipid dependence of phospholipase C in rat liver plasma membranes.
    Author: Momchilova-Pankova AB, Markovska TT, Koshlukova SE, Koumanov KS.
    Journal: J Lipid Mediat; 1991; 3(2):215-23. PubMed ID: 1797153.
    Abstract:
    Investigations have been carried out on the lipid dependence of membrane-bound phosphatidylinositol-specific phospholipase C in rat liver plasma membranes. For this purpose the phospholipid composition of rat liver plasma membranes has been modified in two different ways. The first method included enrichment of plasma membranes with different phospholipids in the presence of lipid transfer proteins, and the second a partial delipidation by means of exogenous phospholipases A2 and C and selective enrichment with different phospholipids. The results indicated that almost all used phospholipids induced activation of phosphatidylinositol-specific phospholipase C except sphingomyelin. Phosphatidylethanolamine and egg yolk phosphatidylcholine were observed to be most effective in phospholipase C activation.
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