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  • Title: Assessment of serological assays for the diagnosis of HIV-1 infections.
    Author: Danaby RS, Constantine NT, Daise RL, Watts DM.
    Journal: J Egypt Public Health Assoc; 1991; 66(5-6):477-90. PubMed ID: 1797961.
    Abstract:
    Many serologic techniques are available for the detection of HIV-1 infection, but their diagnostic performances have been reported to vary depending on the testing situations. We evaluated eight different serologic assays for detecting HIV-1 antibody in sera from suspected high risk groups in North and East Africa. These included: one ELISA bead assay, three indirect ELISAs, two competitive ELISA assays, one gelatin agglutination assay and a recombinant dot-blot assay. A panel of 38 HIV-1 antibody confirmed positive human sera and a panel of 60 HIV negative sera were tested. All sera yielding discordant results were retested for verification of reactivity. Among the different assays evaluated for detecting HIV-1 antibody, sensitivities ranged from 0.919 to 0.974 and test efficiencies ranged from 94.2-98.9%. All assays failed to detect at least one western blot confirmed positive serum; three tests (Organon, DuPont recombinant and Serodia) produced only one false-negative result. Five of the eight assays did not produce any false-positive results. The Organon viral lysate and the DuPont recombinant ELISAs exhibited the best overall performance (test efficiency = 98.9%).
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