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  • Title: A two-step model for acetylcholine control of exocytosis via nicotinic receptors.
    Author: Arnáiz-Cot JJ, de Diego AM, Hernández-Guijo JM, Gandía L, García AG.
    Journal: Biochem Biophys Res Commun; 2008 Jan 18; 365(3):413-9. PubMed ID: 17981151.
    Abstract:
    The view that Ca(2+) entry through voltage-dependent Ca(2+) channels (VDCC) and through nicotinic receptors for acetylcholine (nAChRs) causes equal catecholamine release responses in chromaffin cells, was reinvestigated here using new protocols. We have made two-step experiments consisting in an ACh prepulse followed by a depolarizing pulse (DP). In voltage-clamped bovine chromaffin cells an ACh prepulse caused a slow-rate release but augmented 4.5-fold the much faster exocytotic response triggered by a subsequent depolarizing pulse (measured with capacitance and amperometry). If the ACh prepulse was given with mecamylamine or in low external Ca(2+), the secretion increase disappeared. This suggests a two-step model for the effects of ACh: (1) meager Ca(2+) entry through nAChRs mostly serves to keep loaded with vesicles the secretory machine; and (2) in this manner, the cell is prepared to respond with an explosive secretion of catecholamine upon depolarization and fast high Ca(2+) entry through VDCC.
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