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  • Title: [Investigation of the variation of Cry j 2 concentration in pollen among sugi (Cryptomeria japonica d. Don) trees using a newly established extraction method].
    Author: Goto-Fukuda Y, Yasueda H, Saito A, Kondo T.
    Journal: Arerugi; 2007 Oct; 56(10):1262-9. PubMed ID: 17982287.
    Abstract:
    BACKGROUND: Selection of low-allergen trees is under consideration for the reduction of Sugi (Cryptomeria japonica) pollinosis. The extraction method of Cry j 2 was established and then the variation of Cry j 2 concentrations in pollen among Sugi trees was investigated. METHODS: The extraction efficiency of Cry j 2 was compared among buffers with different pH, ionic species and NaCl concentration. The Cry j 2 concentration in the pollen of 140 trees was measured using the most efficient buffer. RESULTS: NaHCO3 (0.125 M) + 0.5 M NaCl buffer showed the highest Cry j 2 extraction efficiency. The mean and standard deviation of Cry j 2 concentration among 140 trees was 404 +/- 189 microg/g pollen, which was equivalent to that of Cry j 1 (435 +/- 169 microg). CONCLUSION: We revealed that the variation of Cry j 2 concentrations was as large as that of Cry j 1 in the pollen among trees using the newly developed extraction method.
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