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  • Title: Synthesis of 5-fluoropyrimidine nucleotides as sensitive NMR probes of RNA structure.
    Author: Hennig M, Scott LG, Sperling E, Bermel W, Williamson JR.
    Journal: J Am Chem Soc; 2007 Dec 05; 129(48):14911-21. PubMed ID: 17990877.
    Abstract:
    Enzymatic synthesis methods for the fluorinated 5'-triphosphate analogues 5F-UTP and 5F-CTP have been developed to facilitate 19F-labeling of RNAs for biophysical studies. HIV-2 TAR RNAs were synthesized using these analogues by in vitro transcription reactions using T7 RNA polymerase. The uniform incorporation of 5F-U or 5F-C analogues into HIV-2 TAR RNA transcripts does not significantly alter the RNA structure or thermodynamic stability. Fluorine observed homonuclear 19F-19F and heteronuclear 19F-1H NOE experiments providing selective distance information are presented and discussed. The availability of efficient synthesis of 5F-UTP, and for the first time, 5F-CTP, will facilitate the use of 5F-labeled RNAs in structural, ligand binding, and dynamic studies of RNAs using the advantages of 19F-labeling.
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