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  • Title: [Determination of three alkaloids in total alkaloids exfracts from Lotus plumule by RP-HPLC].
    Author: Zhao LB, Jin Y, Wang JL.
    Journal: Zhongguo Zhong Yao Za Zhi; 2007 Sep; 32(17):1768-70. PubMed ID: 17992997.
    Abstract:
    OBJECTIVE: To develop a RP-HPLC method for determination of three alkaloids in total alkaloids exfracted from Lotus plumule. METHOD: A Hypersil C18 column was used with the mobile phase of methanol-phosphate (73:27, pH 9.0) at the detection wavelength of 230 and 286 nm. The flow rate was 1 mL min(-1). RESULT: The linear range was 20-700 microg mL(-1), the recovery of three alkaloids were 97.9%, 98.4% and 98.1%. The RSD of intra-day and inter-day was 0. 24% -4. 83%. Different groups of samples were determined by this method. The result showed that the extraction method with methanol was prior to the extraction method with alcohol. CONCLUSION: The method is simple, rapid and suitable for the determination of three alkaloids in total alkaloids exfracted from L. plumule.
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