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  • Title: Simultaneous uptake of galactose and glucose by Azotobacter vinelandii.
    Author: Wong TY, Murdock CA, Concannon SP, Lockey TD.
    Journal: Biochem Cell Biol; 1991; 69(10-11):711-4. PubMed ID: 1799437.
    Abstract:
    Azotobacter vinelandii growing on galactosides induced two distinct permeases for glucose and galactose. The apparent Vmax and Km of the galactose permease were 16 nmol galactose/min per 10(10) cells and 0.5 mM, respectively. The apparent Vmax and Km of the glucose permease were 7.8 nmol glucose/min per 10(10) cells and 0.04 mM, respectively. Excess glucose had no effect on the galactose uptake. However, excess galactose inhibited glucose transport. The galactosides-induced glucose permease also exhibited different uptake kinetics from that induced by glucose.
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